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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Catalog #	I1904-67U
Applications	Suitable for use in Immunoblot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:5000-1:25,000. 1.0ug of Goat IgG was used in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Rabbit
Source	Mouse
Concentration	~0.7mg/ml
Form	Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Alkaline Phosphatase (MW: 140kD).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgG whole molecule
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed
against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.