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You are here:Home » Antibodies » Abs to IgG2 » Anti -IgG2

Anti -IgG2

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH FC
Catalog #I1904-80H
ApplicationsSuitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:25,000
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase prior to staining.
Optimal dilution determined by the researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Recommended Negative ControlI1904-78R: IgG1 Murine Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9A175
HostMouse
SourceCanine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenCanine IgG
SpecificityRecognizes canine IgG2 heavy chain. Does not react with canine IgG1, IgA or IgM. Species Crossreactivity: rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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