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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH FC

Catalog #	I1904-80H
Applications	Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:5000-1:25,000
	Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase prior to staining.
	Optimal dilution determined by the researcher.
Hybridoma	Spleen cells from immunized BALB/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Recommended Negative Control	I1904-78R: IgG1 Murine Negative Control
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9A175
Host	Mouse
Source	Canine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH7.2, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Canine IgG
Specificity	Recognizes canine IgG2 heavy chain. Does not react with canine IgG1, IgA or IgM. Species Crossreactivity: rabbit
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.