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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH IC

IgG is a glycoprotein that is produced by plasma cells in response to an immunogen (1). It is the only class of immunoglobulins that can cross the placenta, which is mediated by a receptor on placental cells in the Fc region (1). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, -B, and -A/B, which differ by the disulfide connectivity at the hinge region (2). IgG2-A is the known classic structure for the IgG2 subclass defined by structurally independent Fab domains and hinge region (3). IgG2-B is a structure defined by a symmetrical arrangement of a (C(H)1-C(L)-hinge)(2) complex with both Fab regions covalently linked to the hinge (3). IgG2-A/B represents an intermediate form, defined by an asymmetrical arrangement involving one Fab arm covalently linked to the hinge through disulfide bonds (3).
Catalog #	I1904-80L
Applications	Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:10,000-1:20,000
	Immunohistochemistry: 1:500-1:1000
	Immunocytochemistry: 1:50-1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Clone No	10J104
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to residues in human IgG2.
Specificity	Recognizes human IgG2 at ~52kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.