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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Catalog #	I1904-83L
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunodiffusion and Conjugation. Other applications not tested.
Recommended Dilutions	ELISA: 1:40,000-1:200,000
	Western Blot: 1:4000-1:20,000
	Immunohistochemistry: 1:2000-1:10,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgG2a heavy chain
Specificity	Recognizes mouse IgG2a. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, mouse serum and mouse IgG2a. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.