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You are here:Home » Antibodies » Abs to IgG2 » Anti -IgG2a, X-Adsorbed (FITC)

Anti -IgG2a, X-Adsorbed (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Catalog #I1904-83T
ApplicationsSuitable for use in ELISA (Fluorescent) (Fluorescent), Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:1000 -1:5000
ELISA (Fluorescent): 1:10,000- 1:50,000
Optimal dilutions to be determined by researcher.
Label Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 3.6 moles FITC per mole of rabbit IgG
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
PurityPrepared by immunoaffinity chromatography.
ImmunogenMouse IgG2a heavy chain
SpecificityRecognizes mouse IgG2a heavy chain. Crossreactivity against other mouse or human heavy or light chain isotypes is <1%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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