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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IF

Catalog #	I1904-83T
Applications	Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Immunofluorescence: 1:1000 -1:5000
	FLISA: 1:10,000- 1:50,000
	Optimal dilutions to be determined by researcher.
Label	Fluorescein isothiocyanate (FITC)
	Molecular Weight: 390D
	Absorption Wavelength: 495nm
	Emission Wavelength: 528nm
	Fluorochrome/Protein Ratio: 3.6 moles FITC per mole of rabbit IgG
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml (UV280)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Purity	Prepared by immunoaffinity chromatography.
Immunogen	Mouse IgG2a heavy chain
Specificity	Recognizes mouse IgG2a heavy chain. Crossreactivity against other mouse or human heavy or light chain isotypes is <1%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.