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You are here:Home » Antibodies » Abs to IgG2 » Anti -IgG2b

Anti -IgG2b

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catalog #I1904-84B
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunodifusion and Conjugation. Other applications not tested.
Recommended DilutionELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG2b
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing protein added.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG2b gamma 2b chain
SpecificityRecognizes mouse IgG2b. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, mouse serum and mouse IgG. Crossreacts with mouse IgG1: 4.0%. Does not crossreact with mouse IgG2a or mouse IgG3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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