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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH Gel

Catalog #	I1904-84B
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunodifusion and Conjugation. Other applications not tested.
Recommended Dilution	ELISA: 1:20,000-1:100,000
	Western Blot: 1:2000-1:10,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG2b
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing protein added.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Mouse IgG2b gamma 2b chain
Specificity	Recognizes mouse IgG2b. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, mouse serum and mouse IgG. Crossreacts with mouse IgG1: 4.0%. Does not crossreact with mouse IgG2a or mouse IgG3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.