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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Catalog #	I1904-84E
Applications	Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Immunofluorescence: 1:500-1:2500
	Optimal working dilutions to be determined by researcher.
Label	Fluorescein isothiocyanate (FITC)
	Molecular Weight: 390D
	Absorption Wavelength: 495nm
	Emission Wavelength: 528nm
	Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Rabbit IgG
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml (UV280)
Form	Supplied as a lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute in sterile 40-50% glycerol, dH2O.
Purity	Prepared by immunoaffinity chromatography.
Immunogen	Mouse IgG2b.
Specificity	Recognizes mouse IgG2b, g2b chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.