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You are here:Home » Antibodies » Abs to IgG2 » Anti -IgG2b (FITC)

Anti -IgG2b (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Catalog #I1904-84E
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:500-1:2500
Optimal working dilutions to be determined by researcher.
Label Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Rabbit IgG
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute in sterile 40-50% glycerol, dH2O.
PurityPrepared by immunoaffinity chromatography.
ImmunogenMouse IgG2b.
SpecificityRecognizes mouse IgG2b, g2b chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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