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You are here:Home » Antibodies » Abs to IgG3 » Anti -IgG3 (FITC)

Anti -IgG3 (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E FC IF
Catalog #I1904-85F
ApplicationsSuitable for Immunomicroscopy and Flow Cytometry. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Label Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 2.8 moles FITC per mole of Rabbit IgG
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label with Fluorescein isothiocyanate (FITC). Reconstitute in sterile dH2O or 40-50% glycerol.
PurityPrepared by immunoaffinity chromatography.
ImmunogenMouse IgG3
SpecificitySpecific for Mouse IgG3, gamma 3 chain. Crossreactivity against other mouse heavy or light chain isotypes is < 1%. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum and mouse serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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