Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to IgG3 » Anti -IgG3 (HRP)

Anti -IgG3 (HRP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-85H
ApplicationsSuitable for Western or Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:31,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)(after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.15M sodium chloride, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse IgG3 heavy chain.
SpecificityReconizes Mouse IgG3. Assay by IEP resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links