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You are here:Home » Antibodies » Abs to IgG3 » Anti -IgG3 (AP)

Anti -IgG3 (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #I1904-85J
ApplicationsSuitable for Immunoblotting (Western or Dot Blot), ELISA and immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:2000-1:4000 Use pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT.
Optimal dilution determined by the researcher.
LabelAlkaline Phosphatase (Calf Intestine) (MW 140kD)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphotase (AP).
PurityPurified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.
ImmunogenMouse IgG3 heavy chain
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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