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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains-two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. There are 4 IgG isotypes, IgG1, IgG2, IgG3 and IgG4. IgG4 makes up 4% of total IgG.
Catalog #	I1904-89
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8.F.178
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human IgG4.
Specificity	Recognizes human IgG4 (kappa and lambda light chain). Fc region specific. No crossreactivty was found against IgG1, IgG2, IgG3, IgM, IgA and IgD. Determined by hemagglutination of sheep red blood cells coated with purified human myeloma immunoglobulin, using chromic chloride. A positive reaction occurred with cells coated with IgG (Fc portion), polyclonal IgG and IgG4 (kappa or lambda light chain). Negative reaction occurred with cells coated with IgG1, IgG2, IgG3, free kappa or lambda, IgM, IgA1 (lambda), IgA2 (kappa) and IgD (lambda).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.