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You are here:Home » Antibodies » Abs to IgM » Anti -IgM (AP)

Anti -IgM (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1905-01D
ApplicationsSuitable for use in Immunomicroscopy, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:4500-1:8000
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~1-2mg/ml
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA, (IgG and Protease free) 0.1% sodium azide, 50% glycerol. Labeled with alkaline phosphatease (AP).
PurityPurified by immunoaf nity chromatography.
ImmunogenMouse IgM mu heavy chain.
SpecificityRecognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, mouse IgM and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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