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You are here:Home » Antibodies » Abs to IgM » Anti -IgM (Biotin)

Anti -IgM (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1905-01S
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested.
Recommended DilutionsELISA: 1:20,000-1:100,000
Immunohistochemistry: 1:1000-1:5000
Western Blot: 1:2000-1:10,000
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
Concentration~1mg/ml (UV280) (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenRat IgM mu chain.
SpecificityRecognizes Rat IgM mu chain. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgM and rat serum. No reaction was observed against rat IgA or rat IgG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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