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You are here:Home » Antibodies » Abs to IgM » Anti -IgM (AP)

Anti -IgM (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #I1905-02C2
ApplicationsSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:10,000
Western Blot: 1:500-1:3000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Clone TypePolyclonal
IsotypeIgM
HostGoat
SourceMonkey
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide 50% glycerol, pH 8.0. Labeled with Alkaline Phosphatase (AP), MW: 140kD.
PurityPurified by immunoaffinity chromatography.
ImmunogenMonkey IgM mu heavy chain.
SpecificityRecognizes monkey IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, monkey IgM and monkey serum. Cross-reacts against other mouse or human heavy or light chain isotypes; <1%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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