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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	I1905-02C2
Applications	Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:2000 to 1:10,000 using 1ug Monkey IgM in a standard capture ELISA using pNPP for 30 minutes at RT.
	Western Blot: 1:500- 1:3000
	Immunohistochemistry: 1:200- 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgM
Host	Goat
Source	Monkey
Concentration	~1mg/ml (UV280)
Form	Supplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide 50% glycerol, pH 8.0. Labeled with Alkaline Phosphatase (Calf Intestine, 140kD).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Monkey IgM mu heavy chain.
Specificity	Recognizes monkey IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, monkey IgM and monkey serum. Less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.