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You are here:Home » Antibodies » Abs to IgY » Anti -IgY, Fc (HRP)

Anti -IgY, Fc (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #I1906-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:100,000; Assayed against 1ug/ml chicken IgY. This dilution produced an O.D. = 1.0 in a 15 minute reaction with TMB Substrate.
Western Blot (Colorimetric): 1:2000-1:4000
Western Blot (ECL): 1:10,000
Optimal dilutions to be determined by the researcher
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceChicken
Concentration~1mg/ml
FormSupplied as a liquid in 50mM HEPES, pH 7.0, 0.1M sodium chloride, 0.2% BSA, 0.01% Thimerosal. Conjugated to horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified chicken IgY Fc fragment isolated from normal chicken yolk IgY.
SpecificityRecognizes chicken IgY. Does not crossreact with other chicken immunoglobulins. Species Crossreactivity: Does not crossreact with other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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