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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Catalog #	I1906-01
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:100,000; Assayed against 1ug/ml chicken IgY. This dilution produced an O.D. = 1.0 in a 15 minute reaction with TMB Substrate.
	Western Blot (Colorimetric): 1:2000-1:4000
	Western Blot (ECL): 1:10,000
	Optimal dilutions to be determined by the researcher
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Chicken
Concentration	~1mg/ml
Form	Supplied as a liquid in 50mM HEPES, pH 7.0, 0.1M sodium chloride, 0.2% BSA, 0.01% Thimerosal. Conjugated to horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Highly purified chicken IgY Fc fragment isolated from normal chicken yolk IgY.
Specificity	Recognizes chicken IgY. Does not crossreact with other chicken immunoglobulins. Species Crossreactivity: Does not crossreact with other species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.