Anti -IkB alpha, phosphorylated (Ser32/Ser36) (NF-kappa-B Inhibitor alpha,
I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major Histocompatibility Complex
Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
IH |
|
| The NF-kB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkB proteins. Activation of IkB-a occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser32 and Ser36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-kB. This pathway of IkB-a regulation occurs in response to various NF-kB-activating agents, such as TNFa, interleukins, LPS, and irradiation. An alternative pathway for IkB-a regulation occurs through tyrosine phosphorylation of Tyr42 and Tyr305. Tyr42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IkB-a and NF-kB-activation. In contrast, Tyr305 phosphorylation by c-Abl has been implicated in IkB-a nuclear translocation and inhibition of NF-kB-activation. Thus, tyrosine phosphorylation of IkB-a may be an important regulatory mechanism in NF-kB signaling. | | | Catalog # | I2500-03B | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic phosphopeptide corresponding to human IkB-a around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K). | | Specificity | Recognizes human IkBa, phosphorylated at Ser32/Ser36. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
