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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IkB alpha (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI)

Anti -IkB alpha (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha,
Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkB proteins. Activation of IkB-a occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser32 and Ser36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-kB. This pathway of IkB-a regulation occurs in response to various NF-kB-activating agents, such as TNFa, interleukins, LPS, and irradiation. An alternative pathway for IkB-a regulation occurs through tyrosine phosphorylation of Tyr42 and Tyr305. Tyr42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IkB-a and NF-kB-activation. In contrast, Tyr305 phosphorylation by c-Abl has been implicated in IkB-a nuclear translocation and inhibition of NF-kB-activation. Thus, tyrosine phosphorylation of IkB-a may be an important regulatory mechanism in NF-kB signaling.
Catalog #I2500-03B1
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlMCF7 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human IkB-a around the phosphorylation site of serine 32/36 (H-D-SP-G-L- D-SP -M-K).
SpecificityRecognizes human IkBa. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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