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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IkB alpha (I-kappa-B alpha, I(kappa)B(alpha), IkappaBalpha, IkB-alpha, IkBa, Major Histocompatibility Complex Enhancer Binding Protein MAD3, MAD-3, NF-kappa-B Inhibitor alpha, NFKBIa, NFKBI, Nuclear Factor of kappa Light Chain Gene Enhancer in B cells, Nu

Anti -IkB alpha (I-kappa-B alpha, I(kappa)B(alpha), IkappaBalpha, IkB-alpha, IkBa, Major
Histocompatibility Complex Enhancer Binding Protein MAD3, MAD-3, NF-kappa-B Inhibitor
alpha, NFKBIa, NFKBI, Nuclear Factor of kappa Light Chain Gene Enhancer in B cells, Nu

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.
Catalog #I2500-03K
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Positive Controlanti-Fas treated Jurkat (30 min)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9G89
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the caspase-3 mediated cleavage site of IkBa.
SpecificitySpecies Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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