Anti -IkB alpha, phosphorylated (Tyr42) (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha,
IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3,
NFKBIA, IKBA, MAD3, NFKBI)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
E |
|
| NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a , IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. One of the first genes induced following NF-kB activation is IkBa. | | | Catalog # | I2500-05B | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilution | ELISA: 1:100-1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide. | | Purity | Purified. | | Immunogen | Synthetic phosphopeptide around Tyr42 corresponding to aa34-48 (LDSMKDEEY*EQMVKE) of human IkBa; (Genbank accession no. NP_065390.1.) (Y* refers-phosphorylated tyrosine.) | | Specificity | Recognizes human IkBa when phosphorylated at Tyr42. Species Crossreactivity: monkey, mouse, porcine, bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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