Anti -IkB alpha, phosphorylated (Tyr42) (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha,
IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3,
NFKBIA, IKBA, MAD3, NFKBI)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
|
| The NF-kB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkB proteins. Activation of IkBa occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser32 and Ser36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-kB. This pathway of IkBa regulation occurs in response to various NF-kB-activating agents, such as TNFa, interleukins, LPS, and irradiation. An alternative pathway for IkBa regulation occurs through tyrosine phosphorylation of Tyr42 and Tyr305. Tyr42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IkBa and NF-kB-activation. In contrast, Tyr305 phosphorylation by c-Abl has been implicated in IkBa nuclear translocation and inhibition of NF-kB-activation. Thus, tyrosine phosphorylation of IkBa may be an important regulatory mechanism in NF-kB signaling. | | | Catalog # | I2500-05F | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Immunoprecipitation: 5-10ul | | Optimal dilutions to be determined by the researcher. | | Positive Control | Pervanadate treated A431 or Jurkat cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% gylcerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa residues around tyrosine 42 of human IkBa (KLH). | | Specificity | Recognizes human IkBa phosphorylated at Tyr42. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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