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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IkB alpha (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI)

Anti -IkB alpha (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha,
Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
NF-kB is a family of transcription factors, including p50, p52, p65, RelB, and C-Rel. It regulates the expression of a number of proteins involved in the regulation of cell survival and immune responses. The biological activity of NF-kB is tightly controlled by its inhibitor protein IkB, which binds to and sequesters NF-kB in the cytoplasm. There are three isoforms of the IkB: IkBa, -b and -e. IkBa, the well-characterized member of the IkB family, contains both nuclear localization sequence and nuclear export signal that render to IkBa the activity to rapidly shuttle between the nucleus and the cytoplasm. The IkBa protein binds to NF-kB dimers, masking their nuclear localization sequences and causing their cytoplasmic retention. After NF-kB positive stimuli, IkB kinase complex phosphorylates IkBs in two conserved serines (Ser-32 and Ser- 36 in IkBa), leading to ubiquitination and subsequent proteasomal degradation.
Catalog #I2500-05H
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide (RHDSGLD) corresponding to human IkBa around serines 32 and 36.
SpecificityRecognizes human IkBa.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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