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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IKBE, phosphorylated (Ser22) (NFKBIE, NF-kappa-B Inhibitor epsilon, NF-kappa-BIE, I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon)

Anti -IKBE, phosphorylated (Ser22) (NFKBIE, NF-kappa-B Inhibitor epsilon, NF-kappa-BIE,
I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
IkB-e is a novel member of the I kappaB family which associate with the p50 subunit of NF-kappaB, regulating NF-kappaB-dependent transcription. IkB-e interacts with different NF-kappaB proteins, including p65 (RelA), c-Rel, p50, and p52, in vitro and in vivo and inhibits the DNA-binding activity of both p50-p65 and p50-c-Rel complexes effectively. IkB-e mRNA is widely expressed in different human tissues, with highest levels in spleen, testis, and lung. It is also synthesized constitutively in transformed B-cell lines. The protein has six consecutive ankyrin repeats. Human IkB-e has been mapped to chromosomal region 6p21.1.
Catalog #I2500-25B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control293 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human IkB-e around the phosphorylation site of serine 22 ( I-E-SP-L-R).
SpecificityRecognizes human IkBe, phosphorylated at Ser22. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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