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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IKK alpha (IkappaB Kinase alpha, IKKa, CHUK, Conserved Helix Loop Helix Ubiquitous Kinase, CHUK1, IkappaB Kinase 1, IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor kappa B Kinase alpha, NFKBIKa, Nucl

Anti -IKK alpha (IkappaB Kinase alpha, IKKa, CHUK, Conserved Helix Loop Helix Ubiquitous
Kinase, CHUK1, IkappaB Kinase 1, IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene
Enhancer In B Cells, Inhibitor Of Nuclear Factor kappa B Kinase alpha, NFKBIKa, Nucl

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for the NF-kB activation. IKKa specifically phosphorylates IkB-a. IKKa is expressed in a variety of human tissues.
Catalog #I3000-01
ApplicationsSuitable for use in Immunoblot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoblot: 0.5-2ug/ml detected IKKa/IKK1in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with I3000-01 (0.5ug/ml). Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
Immunoprecipitation: 8ug immunoprecipitated IKKa/IKK1 from a HeLa RIPA lysate.
Optimal dilutions to be determined by researcher.
Positive Antigen Control J9000
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.156
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHis-tagged, full length human IKKa/IKK1
SpecificityRecognizes IKKa/IKK1, Mr 85kD. Species Crossreactivity: Human, mouse and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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