Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IKK alpha, phosphorylated (Thr23) (IKKA, Inhibitor of Nuclear Factor kappa-B Kinase Subunit alpha, I-kappa-B Kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB Kinase, Conserved Helix-loop-helix Ubiquitous Kinase, I-kappa-B Kinase 1, IKK1, Nuclear Factor NF-k

Anti -IKK alpha, phosphorylated (Thr23) (IKKA, Inhibitor of Nuclear Factor kappa-B Kinase
Subunit alpha, I-kappa-B Kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB Kinase, Conserved
Helix-loop-helix Ubiquitous Kinase, I-kappa-B Kinase 1, IKK1, Nuclear Factor NF-k

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for the NF-kB activation. IKKa specifically phosphorylates IkB-a. IKKa is expressed in variety of human tissues.
Catalog #I3000-02E
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human IKK a around the phosphorylation site of threonine 23 (L-G-TP-G-G).
SpecificityRecognizes human IKKa, phosphorylated at Thr23. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links