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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IKK alpha (IkappaB Kinase alpha, IKKa, CHUK, Conserved Helix Loop Helix Ubiquitous Kinase, CHUK1, IkappaB Kinase 1, IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor kappa B Kinase alpha, NFKBIKa, Nucl

Anti -IKK alpha (IkappaB Kinase alpha, IKKa, CHUK, Conserved Helix Loop Helix Ubiquitous
Kinase, CHUK1, IkappaB Kinase 1, IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene
Enhancer In B Cells, Inhibitor Of Nuclear Factor kappa B Kinase alpha, NFKBIKa, Nucl

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1) (1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns (2,3).
Catalog #I3000-04K
Positive Control Daudi, NIH 3T3, or Raw
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8L368
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenThis antibody was raised against a His-tagged full-length human IKKa/IKK1 protein.
SpecificityAn 85kD band should be observed. Species Crossreactivity: Human, Monkey, Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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