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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IKK alpha, phosphorylated (Ser176, Ser180) (IKKa, IKK1, IkappaB Kinase alpha, Conserved Helix Loop Helix Ubiquitous Kinase, CHUK1, IkappaB Kinase 1, IkB Kinase alpha, IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of

Anti -IKK alpha, phosphorylated (Ser176, Ser180) (IKKa, IKK1, IkappaB Kinase alpha,
Conserved Helix Loop Helix Ubiquitous Kinase, CHUK1, IkappaB Kinase 1, IkB Kinase alpha,
IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #I3000-07M
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1.5ug/ml. at 0.75ug/ml, the dilution provides 100ml workig soluion, which 10ml/blot allows 10 blots to be performed. When examining phosphorylation of endogenous IKKa protein, we recommend that IKKa first be immunoprecipitated.
Optimal dilutions to be determined by the researcher
Positive ControlImmunoprecipitates prepared from lysates of Jurkat cells, treated with TNF-a.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, before tha addition of glycerol to 40%.
PurityPurified by affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of human IKKa that contains serine 176 and serine 180.
SpecificityRecognizes human IKKa when phosphorylated at serines 176 and 180. Species sequence homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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