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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IKK beta (IkappaB Kinase beta, IKKb, IKK2, IkBKb, IkappaB Kinase 2, Inhibitor of kappa Light Chain Gene Enhancer in B cells, Inhibitor of kappa Light Polypeptide Gene Enhancer in B cells Kinase beta, Inhibitor of Nuclear Factor kappa B Kinase beta, MGC131

Anti -IKK beta (IkappaB Kinase beta, IKKb, IKK2, IkBKb, IkappaB Kinase 2, Inhibitor of
kappa Light Chain Gene Enhancer in B cells, Inhibitor of kappa Light Polypeptide Gene
Enhancer in B cells Kinase beta, Inhibitor of Nuclear Factor kappa B Kinase beta, MGC131

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP FC
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).
Catalog #I3000-08H
Positive Control Daudi, HeLa, NIH 3T3
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L372
HostMouse
SourceHuman
ConcentrationAs reported
FormPBS containing 0.2% gelatin, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA full-length human IKKb recombinant protein was used to immunize mice.
SpecificityAn ~87kD band should be observed. Species Crossreactivity: Human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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