Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -IKKB (Inhibitor Of Nuclear Factor kappa B Kinase beta Subunit, I-kappa-B-kinase beta, IkBKB, IKK-beta, IKK-B, I-kappa-B Kinase 2, IKK2, Nuclear Factor NF-kappa-B Inhibitor Kinase beta, NFKBIKB, IKBKB)

Anti -IKKB (Inhibitor Of Nuclear Factor kappa B Kinase beta Subunit, I-kappa-B-kinase
beta, IkBKB, IKK-beta, IKK-B, I-kappa-B Kinase 2, IKK2, Nuclear Factor NF-kappa-B
Inhibitor Kinase beta, NFKBIKB, IKBKB)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP FC
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).
Catalog #I3000-24F
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.25-0.5ug/10e6 cells.
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlDaudi, HeLa, NIH 3T3
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No11E226
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to full-length human IKKb protein.
SpecificityRecognizes human IKKb. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links