You are here:Home
»
Biochemicals
»
Biochem-Biological Buffers
»
Imidazole (1,3-Diazo-2,4-cyclopentadiene; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazole)
Imidazole (1,3-Diazo-2,4-cyclopentadiene; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazole)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Imidazole can be used for the elution of histidine containing proteins from divalent cation resins. | | | Catalog # | I7000 | | Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is also a chelator for the binding of various divalent cations. | | The logs of the binding constants for various divalent cations are below | Divalent Cation Log binding constant | | Ca2+ 0.1 | | Mn2+ 1.6 | | Fe2+ 3.3 | | Co2+ 2.4 | | Ni2+ 2.9 | | Cu2+ 4.2 | | Zn2+ 2.0 | | Solubility (10%) | Colorless, clear, complete | | Imidazole is soluble in dH2O (~500mg/ml). | | Boiling Point | ~256°C | | Melting Point | 88-92°C | | Storage and Stability | Powder may be stored at 4°C. Stable for 12 months at -20°C. Reconstitute with ddH2O Aliquot and store at 4°C. Reconstituted product is stable for 12 months at -4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light. | | CAS Number | 288-32-4 | | Molecular Formula | C3H4N2 | | Molecular Weight | 68.08 | | Purity | ~99% | | Form | Colorless to pale yellow crystalline flakes | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
