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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -Immunoglobulin Superfamily Member 3 (IGSF3, EWI-3)

Anti -Immunoglobulin Superfamily Member 3 (IGSF3, EWI-3)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
IGSF3 (immunoglobulin superfamily member 3; also EWI-3) is a member of the EWI subfamily of the Ig-Superfamily of molecules. It is a type I transmembrane protein that likely exists as a homodimer in kidney and lung. The mature molecule is 1195 amino acids (aa) in length, and contains an extended 1127 aa extracellular domain (ECD) (aa 20-1146). There are seven ECD V-type Ig-like domains, the second of which exhibits a characteristic Glu-Trp-Ile motif. There are at least two potential splice variants. One shows a truncation after Pro833; a second shows a deletion of aa 408-427. Over aa 20-1125, human IGSF3 shares 92% aa identity with mouse IGSF3.
Catalog #I7502-95
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml detection limit for rhIGSF3 is~ 1ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhIGSF3 is ~ 2ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rhIGSF3. (aa 20 - 1125)
SpecificityRecognizes human IGSF3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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