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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -Immunoglobulin Superfamily Member 4C (IGSF4C, Nectin-Like 4, TSLC1-Like Protein 2, TSLL2)

Anti -Immunoglobulin Superfamily Member 4C (IGSF4C, Nectin-Like 4, TSLC1-Like Protein 2,
TSLL2)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC
IGSF4C (Immunoglobulin Superfamily member 4C), also known as Nectin-like 4 (Necl-4) and TSLL2, is a 48-55kD type I transmembrane glycoprotein that is a member of the nectin-like family of IGSF molecules. It is expressed in the brain, kidney, bladder and prostate. IGSF4C forms cis homophilic dimers on cell surfaces and mediates cell adhesion through homophilic trans-interactions in a Ca 2+/Mg 2+-independent manner. IGSF4C may also be a tumor-suppressor candidate in human prostate cancer. The mature 364 amino acid (aa) molecule contains a 299 aa extracellular domain (ECD) (aa 25-323) with three Ig-like motifs and a short cytoplasmic domain. There is at least one potential splice variant, which shows an insertion of 15 aa after Tyr309.
Catalog #I7502-B70
ApplicationsSuitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 50ug/ml. Add 10ul of diluted antibody to 1-5x10e5 cells (max. total volume 200ul).
ELISA: 0.5-1ug/ml; Detection limit for recombinant human IGSF4C is ~0.3ng/well.
Western Blot: 0.1-0.2ug/ml; Detection limit for recombinant human IGSF4C is ~5ng/lane (reducing and non-reducing conditions).
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1903-12S, IgG, H&L (PE) Donkey x Goat
I1903-30A, IgG, H&L, X-Adsorbed (FITC) Rabbit x Goat
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol in PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 0.5ml sterile 40-50% glycerol in PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived recombinant human IGSF4C extracellular domain (aa25-323) Accession # NP_660339
SpecificityRecognizes human lGSF4C. In direct ELISAs and Western Blots, shows ~10% crossreactivity with recombinant human IGSF4B and less than 1% crossreactivity with recombinant human IGSF4D. Species Sequence Homology: Canine, mouse - 99%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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