| IGSF4C (Immunoglobulin Superfamily member 4C), also known as Nectin-like 4 (Necl-4) and TSLL2, is a 48-55kD type I transmembrane glycoprotein that is a member of the nectin-like family of IGSF molecules. It is expressed in the brain, kidney, bladder and prostate. IGSF4C forms cis homophilic dimers on cell surfaces and mediates cell adhesion through homophilic trans-interactions in a Ca 2+/Mg 2+-independent manner. IGSF4C may also be a tumor-suppressor candidate in human prostate cancer. The mature 364 amino acid (aa) molecule contains a 299 aa extracellular domain (ECD) (aa 25-323) with three Ig-like motifs and a short cytoplasmic domain. There is at least one potential splice variant, which shows an insertion of 15 aa after Tyr309. |
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| Catalog # | I7502-B70 |
| Applications | Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 50ug/ml. Add 10ul of diluted antibody to 1-5x10e5 cells (max. total volume 200ul). |
| ELISA: 0.5-1ug/ml; Detection limit for recombinant human IGSF4C is ~0.3ng/well. |
| Western Blot: 0.1-0.2ug/ml; Detection limit for recombinant human IGSF4C is ~5ng/lane (reducing and non-reducing conditions). |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1903-12S, IgG, H&L (PE) Donkey x Goat |
| I1903-30A, IgG, H&L, X-Adsorbed (FITC) Rabbit x Goat |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol in PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 0.5ml sterile 40-50% glycerol in PBS. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | NS0-derived recombinant human IGSF4C extracellular domain (aa25-323) Accession # NP_660339 |
| Specificity | Recognizes human lGSF4C. In direct ELISAs and Western Blots, shows ~10% crossreactivity with recombinant human IGSF4B and less than 1% crossreactivity with recombinant human IGSF4D. Species Sequence Homology: Canine, mouse - 99%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
