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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)

Anti -Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumors and blocking the IDO pathway may be a potential target for immunotherapy.
Catalog #I7545-01
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500 detects IDO on 10ug of untreated and IFN gamma treated Hela lysates. Untreated and IFN-gamma 24-hour-treated Hela lysates were resolved by electrophoresis, transferred to membrane and probed with I7545-01. Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman IFN-gamma stimulated HeLa cell lysates,
IFN-gamma stimulated human peripheral blood lymphocytes
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No4C2
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G chromatography.
ImmunogenGST fusion protein corresponding to aa78-184 of human Indoleamine 2,3-Dioxygenase (IDO).
SpecificityRecognizes human Indoleamine 2,3-Dioxygenase (IDO), Mr 42kD. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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