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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)

Anti -Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Serum E B
Indoleamine 2,3-dioxygenase (IDO), a monomeric hemoprotein, is the initial and rate-limiting enzyme of the kynurenine pathway of degradation of L-tryptophan. IDO oxidizes the pyrrole moiety of tryptophan,1 causing cell death due to tryptophan starvation.2 It is induced in various types of human cell lines and cell types such as fibroblasts, epithelial cells and macrophages in the presence of IFN- .3,4 Placenta, lung and small intestine show high levels of IDO enzyme activity.5 IDO has been implicated in regulation of feto-maternal tolerance and protection against intracellular and extracellular pathogens. In the human female reproductive tract, endometrial glandular and surface epithelial cells show increasing IDO expression during the course of the menstrual cycle.6 IDO is also implicated in playing immuno-regulation. In vivo, IDO has
been implicated in tolerance to self antigens in non-obese diabetic mice, 7 and as a downstream effector mechanism for the toleance-inducing agent CTLA4-Ig.8 Expression of IDO by tumor cells has also been proposed as a mechanism by which tumors evade the immune system.9 Consistent with this hypothesis, a recent study in mice found a subset of plasmacytoid
deudritic cells (pDCs) in tumor-draining lymph nodes (TDLNs) that expressed immunosuppressive levels of IDO.10
Catalog #I7545-04
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:100-1:200
Western Blot: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Positive Control IFN- -stimmulated human peripheral blood lymphocytes
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 0.1% sodium azide.
PuritySerum
ImmunogenRecombinant to human IDO (Indoleamine-pyrrol 2,3-dioxygenase) protein
SpecificityRecognizes human IDO at 44kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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