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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.
Catalog #I7570-31
ApplicationsSutiable for use in Immunohistchemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): Ab at 1:10-1:25 for 30 min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control Lung
Epitope C-terminus
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide. before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from C-ternimal of mouse iNOS. Cellular Localization: Cytoplasmic
SpecificityRecognizes human iNOS. Species Crossreactivity: Rat and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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