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Specifications

Clone Host Grade Applications Polyclonal Goat Highly Purified E B IH IC IF

Influenza type A viruses are divided into subtypes based on the antigenic differences of two viral surface proteins, hemagglutinin (H) and neuraminidase (N). On infection of the respiratory tract, the hemagglutinin molecule binds to sialic acid-containing receptors on the epithelial cells resulting in endocytosis. Once the virus has been engulfed, the hemagglutinin allows the viral membrane to fuse with the endosomal membrane. Neuraminidase functions to aid viral release from host cells by cleaving terminal sialic acid residues from carbohydrate moieties on the cell surface.Subtype antigenic variations result from a process known as antigenic drift whereby these surface proteins constantly mutate in order to evade the host immune repspone. Subtype A(H1N1) was the cause of Spanish flu that killed ~500,000 people between 1918-1919.
Catalog #	I7650-05E
Applications	Suitable for use in ELISA, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry, Hemagglutination Inhibition and Conjugation purposes. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: Fomailin fixed, paraffin emedded sections.
	Optimal dilutions to be determined by the researcher.
Titer	Indirect Immunofluorescence: >1:1000
	Hemagglutination inhibition: >1:2500
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Concentration	~4-5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.
Purity	Purified IgG fraction to a purity of > 95%.
Immunogen	Influenza A, strain USSR (H1N1).
Specificity	Recognizes Influenza A, USSR. Reacts with purified virions. Does not react with Influenza B, RSV, Para 1-3 or Adeno. Does not react with HEp-2 cells. May react with chicken cellular proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.