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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

The nucleoprotein (NP) of Influenza A virus encapsidates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56.
Catalog #	I7650-81J
Applications	Suitable for use in ELISA. Other applications have not been tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from BALB/c mice.
Source	Ascites
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	8J92
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A chromatography. >90% pure (SDS-PAGE).
Immunogen	Purified Influenza A/New Caledonia/20/99 (H1N1)
Specificity	Recognizes Nucleoprotein (NP) of Influenza A. Does not cross react with nucleoprotein of Influenza B virus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.