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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -ING1 (Inhibitor of Growth 1)

Anti -ING1 (Inhibitor of Growth 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
The ING1 (inhibitor of growth) tumor suppressor protein shares a number of biological functions that are very similar with p53. The human ING1 gene contains three exons and two introns. There are four alternative splice variants produced from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 ING1). A number of studies have concluded that ING1 can influence a number of biological functions, which might be dependent on the variant that is expressed. The ING1 family has been reported to mediate growth arrest, senescence, anchorage-dependent growth and apoptosis, as well as influence chemosensitivity.
Catalog #I7652-49
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects ING1b in nuclear extracts from human brain tumor SNB19 cells. SNB19 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with I7652-49 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunocytochemistry: Positive immunostaining for ING1 in human diploid fibroblast cells fixed with formaldehyde.
Immunoprecipitation: Precipitates ING1 from human fibroblasts.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodyI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5H32
HostMouse
SourceHuman
Concentration~0.3mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenGST fusion protein corresponding to raa70-279 of human ING1b. (AAF07921).
SpecificityRecognizes human ING1a, ING1b and ING1c, predicted MW
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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