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You are here:Home » Antibodies » Abs to Insulin » Anti -Insulin

Anti -Insulin

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It is produced by B cells of the pancreas. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage,formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
Catalog #I7656-01B
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen and paraffin sections): 1:50-1:100. Does not require protein digestio or antigen retrieval using heat treatment.
ELISA: 5ng/ml as a detection reagent.
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1904-65W: IgG, F(abí)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab RbxMo
Recommended IHC Positive Control TissuePancreas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2464
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide. No glycerol added.
PurityPurified by Protein A affinity chromatography.
ImmunogenInsulin conjugated to BSA
SpecificityRecognizes human insulin. Species Crossreactivity: Porcine, rabbit, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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