| Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It is produced by B cells of the pancreas. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage,formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma. |
|
| Catalog # | I7656-01B |
| Applications | Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100. Does not require protein digestio or antigen retrieval using heat treatment. |
| ELISA: 5ng/ml as a detection reagent. |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo |
| Recommended IHC Positive Control Tissue | Pancreas |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2464 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide. No glycerol added. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Insulin conjugated to BSA |
| Specificity | Recognizes human insulin. Species Crossreactivity: Porcine, rabbit, bovine. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
