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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.
Catalog #	I7656-05
Applications	Suitable for use in ELISA (two-site enzyme immunoassay). Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Recommended Pair	Suitable for use as the capture or solid phase antibody.
	Matched to I7656-04 as the detection or conjugate (label) antibody.
Hybridoma	X63-Ag8-653 myeloma cells with spleen cells from BALB/c mice.
Affinity Constant	8.0x10e8 M e-1
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	2B60
Host	Mouse
Source	Human
Concentration	~5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified human insulin.
Specificity	Recognizes human insulin. Species Crossreactivity: human proinsulin, porcine insulin and bovine insulin (30%). No crossreaction with free C-peptide.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.