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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Catalog #	I7656-06A
Applications	Suitable for use in ELISA two-site enzyme immunoassay. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Recommended Pair	Matched to I7656-07 as the detection or conjugate (label) antibody.
Affinity Constant	~7.5 x 10e9
Hybridoma	Hybridization of X63-Ag8–653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	Store at 4°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	1.B.105
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase (AP).
Purity	Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
Immunogen	Purified human insulin.
Specificity	Recognizes human Insulin. Species Crossreactivity: human proinsulin, bovine insulin (30%) and porcine insulin. Does not crossreact wtih free C-peptide.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.