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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum IH IF

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.
Catalog #	I7656-17
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence (FITC): 1:400-1:800 with overnight incubation at 4°C
	Immunohistochemistry: Frozen and formalin-fixed paraffin sections
	Optimal dilutions to be determined by the researcher.
Positive Control	Formalin-fixed paraffin sections of porcine pancreas.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Porcine
Concentration	Not determined
Form	Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH2O. Dilute further in sterile 0.1MPBS, 1% BSA, 0.1% sodium azide.
Purity	Serum
Immunogen	Porcine insulin
Specificity	Recognizes porcine insulin. Does not crossreact with polypeptide hormones unrelated to insulin. Species Crossreactivity: human, rat, ferret.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.