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You are here:Home » Antibodies » Abs to Insulin » Anti -Insulin

Anti -Insulin

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as B-cell and tumor (insulinoma) marker.
Catalog #I7660-16A
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin-fixed/Paraffin): 0.5-1ug/ml for 30 min at RT. No special pretreatment is required for staining.
Optimal dilutions to be determined by the researcher.
Positive ControlPancreas
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note We do not recommended adding glycerol as a cryopreservative.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F210
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See I7660-16AX.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified Insulin protein. Molecular Weight of Antigen
SpecificityRecognizes human Insulin. Excellent for identifying the B-cells of islets of Langerhans in routine formalin-fixed, paraffin-embedded pancreatic tissues. Species Crossreactivity: Porcine and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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