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You are here:Home » Antibodies » Abs to Insulin » Anti -Insulin (BSA & Azide Free)

Anti -Insulin (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Insulin is a 51-amino acid polypeptide (~6kD) composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as b-cell of islets of Langerhans and tumor (insulinoma) marker.
Catalog #I7660-16X
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:25-1:50 for 30 min RT. No special pretreatment required.
Optimal dilutions to be determined by the researcher.
Positive ControlPancreas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F213
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. No glycerol added. Also available with BSA and azide. See I7660-16
PurityPurified by Protein G affinity chromatography.
ImmunogenPorcine insulin. Cellular Localization: Cytoplasmic.
SpecificityRecognizes human Insulin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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