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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

Catalog #	I7660-22
Applications	Suitable for use in ELISA. Other applications have not been tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Hybridoma	Hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
Reactiviy	Kass is 7.5 x 10e9Me-1
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5C54
Host	Mouse
Source	Human
Concentration	~5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% of sodium azide. No glycerol added
Purity	Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
Immunogen	Purified human insulin
Specificity	Recognizes human Insulin. Reacts with C-terminal pentapeptide of insulin beta-chain. Crossreact with human proinsulin, bovine insulin (30%) and porcine insulin. No crossreaction with free C-peptide.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.