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You are here:Home » Antibodies » Abs to Insulin » Anti -Insulin

Anti -Insulin

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse E B IH IC
Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as â-cell of islets of Langerhans and tumor (insulinoma) marker.
Catalog #I7660-22A
Cellular Localization Secreted
Positive Control Pancreas
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8L393
HostMouse
SourcePorcine
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2.
PurityAscites
ImmunogenFull length Swine Insulin protein.
SpecificityThe antibody recognizes the biologically most active forms of insulin on the C terminal end. It does not react with pro-insulin, C-peptide, thyroglobulin, albumin and insulin from rat, rabbit and/or guinea-pig (evaluated by simple-binding ELISA). The antibody labels the cytoplasm of beta cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method). Species Crossreactivity: Crossreacts with Human, bovine, Rabbit, Rat and Pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesINS, Insulin Precursor


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