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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Affinity Purified E

Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
Catalog #	I7660-25D
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	ELISA: 1:3200 (0.3125ug/ml)
	Optimal dilutions to be determined by the researcher.
Amino Acid Sequence	Gly-Ile-Val-Glu-Gln-Cys-Cys-Thr-Ser-Ile-Cys-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn-Phe-Val-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS. Reconstitute with 400ul sterile PBS.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to human Insulin.
Specificity	Recognizes human Insulin. Species Crossreactivity: bovine and porcine. Does not crossreact with human C-Peptide, human, rat, mouse or porcine Somatostatin, or human, rat, mouse, porcine, bovine or canine Glucagon, or human Amylin, Pancreatic polypeptide or IGF-1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.