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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
Catalog #	I7660-30A
Application	Suitable in the detection of rat/mouse insulin/proinsulin in two-site enzyme immunoassay. Can be used in immunohistochemistry on frozen sections. Other applications have not been tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Affinity Constant	6.3x10e-8M
Recommended Pair	Capture: Detection:
	I7660-30B I7660-30A
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	1.B.115
Host	Mouse
Source	Human
Concentration	~3mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol. Also available conjugated to Biotin. See I7660-30A1.
Purity	Chromatography on protein A Sepharose. Purity is tested by electrophoresis.
Immunogen	Purified human insulin.
Specificity	Crossreacts with human, bovine, porcine insulin and proinsulin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.