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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
Catalog #	I7660-30B
Applications	Suitable for use in detection of rat/mouse insulin/proinsulin in two site enzyme immunoassays, Immunohistochemistry and sandwich immunoassay. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry: Frozen sections.
	Optimal dilutions to be determined by the researcher.
Recommended Pair	I7660-30A
Hybridoma	Hybridization of X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice.
Affinity Constant	8.1x10e-8 M
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
	Note: We do not recommended adding glycerol as a cryopreservative.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	1.B.116
Host	Mouse
Source	Human
Concentration	~6mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A chromatography. Purity is tested by electrophoresis.
Immunogen	Purified human insulin
Specificity	Recognizes human, bovine and porcine insulin and proinsulin. Directed to different antigenic determinants.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.