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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites IH

Insulin is a polypeptide hormone that is produced by beta cells in the islets of langerhans glands, located in the pancreas. It controls the level of the sugar glucose in the blood. The insulin mRNA is translated as a single chain precursor called preproinsulin, and removal of its signal peptide during insertion into the endoplasmic reticulum generates proinsulin. Proinsulin consists of three domains: an amino-terminal B chain, a carboxy-terminal A chain and a connecting peptide in the middle known as the C peptide. Within the endoplasmic reticulum, proinsulin is exposed to several specific endopeptidases which excise the C peptide, thereby generating the mature form of insulin. Insulin and free C peptide are packaged in the Golgi into secretory granules which accumulate in the cytoplasm. Apart from its primary role in glucose uptake, it allows the transport ofaa into cells, has anabolic functions like stimulation of glucose oxidation, protein synthesis, RNA synthesis, fatty acid synthesis, and glycosaminoglycan synthesis. It also activates fat formation and helps to improve nitrogen retention.
Catalog #	I7660-30N
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	:
	Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
	Optimal dilutions to be determined by the researcher.
Positive Control	Pancreas
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	11E230
Host	Mouse
Source	Porcine
Concentration	Not determined.
Form	Supplied as a liquid, 0.05% sodium azide.
Purity	Ascites
Immunogen	Swine insulin. Cellular Localization: Cytoplasmic.
Specificity	Recognizes porcine insulin. Species Crossreactivity: bovine, human, rabbit, rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.