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Insulin-Like Growth Factor I, Recombinant, Mouse (Insulin-like Growth Factor 1, Igf1, Igf-1, IGF-I, C730016P09Rik, Somatomedin)
Insulin-Like Growth Factor I, Recombinant, Mouse (Insulin-like Growth Factor 1, Igf1,
Igf-1, IGF-I, C730016P09Rik, Somatomedin)
Pricing
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| Catalog # | I7661-10L | | Source | Recombinant corresponding to 70aa of human Insulin Like Growth Factor-1 expressed in E.coli is a single, non-glycosylated, polypeptide chain. | | Molecular Weight | ~7.6kD | | Biological Activity | The ED50, calculated by the dose-dependent proliferation of mouse BALBC 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10U/mg. | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences(IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~7.6kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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